BS EN 17203:2021 pdf free

BS EN 17203:2021 pdf free.Foodstuffs—Determination of citrinin in food by HPLC-MS/MS.
Add 10 ml of glacial acetic acid (5.3) to 990 ml of water (5.8) and mix (water + glacial acetic acid, 99+ 1, v+v). Dissolve 100 g of sodium chloride (5.6) in 11 of this mixture and add 16 ml of hydrochloric acid solution (5.7). This solution can be used for I month if stored at room temperature.
5.13 Extraction solution 2.
Mix 240 ml of acetonitrile (5.2) with 750 ml of ethyl acetate (5.1) and 10 ml of glacial acetic acid (5.3). This solution (ethyl acetate + acetonitrile + glacial acetic acid, 75+24+ 1, v+v+v) can be used for 1 month if stored at room temperature.
5.14 Dilution solution.
Mix 80 ml of methanol (5.10). 18 ml of water (5.9) and 2 ml of glacial acetic acid (5.4). This solution (methanol + water + glacial acetic acid, 80+18+2, v+v+v) can be used for 1 month if stored at room temperature.
5.15 Ammonium acetate/glacial acetic acid in water.
Dissolve 9,5 g of ammonium acetate (5.11) in 12,5 ml of water (5.9). then add 12,5 ml of glacial acetic acid (5.4) and mix thoroughly. This solution can be used for 12 months if stored at < —18 °C.
5.16 Mobile phase A: ammonium acetate/glacial acetic acid in water, molar concentration c = S mmol/l.
Add 1 ml of ammonium acetate/glacial acetic acid in water (5.15) to 999 ml of water (5.9) and mix thoroughly.
5.17 Mobile phase B: ammonium acetate/glacial acetic acid in methanol, c = 5 mmol/l.
Add 1 ml of ammonium acetate/glacial acetic acid in water (5.15) to 999 ml of methanol (5.10) and mix thoroughly.
5.18 Citrinln, analytical standard> 99 %, e.g. crystalline or as certified standard solution.
5.19 Citnnin stock solution, mass concentration p = 500 ig/ml.
Weigh 5mg of crystalline citrinin to the nearest 0,1 mg into a 10 ml volumetric flask and dissolve with acetonitrile (5.2) by filling up to the mark. The mass concentration of this stock solution shall be checked. This can be achieved via LC-MS/MS analysis against the certified standard solution (5.18) or by a photometric determination of concentration using the molar extinction coefficient [81.
The certified standard solution (5.18) can alternatively be used as stock solution.BS EN 17203 pdf download.